Background. Prostate cancer is the most frequently diagnosed cancer in male. Most frequently used screening method is to measure PSA (Prostate cancer specific antigen), which still cannot give a clear answer to patients having gray zone value of PSA.
Aims. To find more practical non-invasive method for prostate cancer screening, differentially expressed genes between prostate cancer and normal in urine were investigated.
Methods. Gene expression microarray (HT12, illumina) was performed to find differentially expressed genes between prostate cancer tissues and benign prostatic hyperplasia tissues (normal). To quantify selected genes in urine or tissue, digital PCR (QX200, BioRad) was carried out.
Results. After identification of differentially expressed genes using prostate cancer cell line (LnCap) and normal cell line (mononuclear cell) using Illumina gene expression array, we have confirmed and selected 4 over-expressed genes and 4 down-expressed genes between prostate cancer tissue and benign prostatic hyperplasia tissues. These genes were found to be similar in the expression pattern between prostate patient`s urine samples and normal urine samples. Currently, we are confirming those genes’ expression pattern by multiplex digital PCR as potential biomarkers for prostate cancer screening with large number of urine samples. The more results will be presented in the conference.
Summary/conclusions. Expression pattern of specific genes in urine samples can be used as practical and potential biomarker for prostate cancer screening.
