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Aims: We have earlier by TUNEL staining established that overexpression of Wnt7a which is a ligand of the Vangl2 receptor increases apoptosis of Jurkat T-cells compared to control. We now wanted to see how overexpression of Vangl2 affects apoptosis and inflammation. Since wnt7a is a ligand of Vangl2, it is hypothesised that by activating either the canonical or regulating non-canonical pathway, wnt7a is a factor to consider when it comes to bladder cancer.
Methods: Jurkat T-cells were cultured on coverslips in 6-well plates until they became 80% confluent. They were transfected with a commercially bought Vangl2-GFP construct for 24 or 48 hours while the controls were transfected with GFP.
Results: The frequency of TUNEL stained cells were higher among the Jurkat T- cells that were transfected with Vangl2 compared to control cells. We could also see a rearrangement of p53 and Th17 expression which are important in inflammation.
Conclusion: We conclude that Vangl2 overexpression affects apoptosis and hypothise that this is done via caspase-8 signaling. We also suggest that the rearrangement of p53 and Th17 expression is a result of actin translocation from the cytoplasm into the nucleus and might be mediated by exosomes.